An Efficient ventilation configuration of an Airborne Infection Isolation Room (AIIR) is essential for protecting Health care workers (HCW) from exposure to potentially-infectious patient aerosol. This paper presents the Computational Fluid Dynamics (CFD) study to predict airflow distribution patterns throughout the AIIR, air velocity vectors in the HCW’s region, streamline of an air particle from patient’s infectious source and the comfort working conditions for the HCW for a range of AIIR ventilation configuration design challenge.